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A whole-genome shotgun  (9) technique was used, and our coverage  is 5.9\"\u0001 (Xia et al. A Draft Sequence for the Genome  of the Domesticated Silkworm  (Bombyx mori). 2004. Science. 306, 1937-1940  Japanese project: \"Genomic DNA was isolated from posterior silkglands  of 5th instar larvae on day 3 of strain p50T of B. mori.\" Mita et al. The Genome Sequence of Silkworm, Bombyx mori. DNA Research 11, 27\u009635 (2004). Japanese sequencing was performed at Genome Research Department, National Institute of Agrobiological Sciences, 1-2 Owashi, Tsukuba, Ibaraki  305-8634, Japan. For mtDNA: \"A B. mandarina sample was collected in Tsukuba,  Ibaraki, Japan. Bombyx mori C108 strain has been maintained  in the National Institute of Agrobiological Sciences.  The entire mt genomes of B. mandarina and B.  mori C108 strain were PCR-amplified in 13 and 11  overlapping fragments, respectively. 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